Fig. 6. ROS generation in WT and VDAC1^-/- and VDAC1 restored VDAC1^-/-C1 H9c2 cells exposed to tBHP or rotenone with H₂DCFDA staining. (A) WT, VDAC1^-/-C1, C2 and VDAC1^-/-C1+ restored VDAC1 H9c2 cell exposed to 50 μM tBHP. (B) WT, VDAC1^-/-C1 and VDAC1^-/-C1+ restored VDAC1 H9c2 cell exposed to 50 μM rotenone. *P<0.05 vs WT+tBHP, ^#P<0.05 vs VDAC1^-/-C1+tBHP. Each experiment was repeated three times.